Epigenetics

Epigenetics

Microbial Community(प्राणमय कोष) controls Gene expression : Not genes, epigenetic!

http://www.cell.com/cms/attachment/2079859016/2071400130/fx1.jpg
http://www.cell.com/cms/attachment/2079859016/2071400130/fx1.jpg

Instead of medicine, why I suggest all friends to worship Surya and Hanuman! 🙂 It is the need of the hour as our Prana is being consumed daily rapidly by toxic environment, physical and mental.

So many dots can be connected by reading this research paper.

This is wonderful morning as I read this research. One more confirmation proof for my theory that it is प्राणमय कोष that actually influences body and not the genes!!

Entire Genome project is pseudo-science of last century! And so GMO crops! Yes, this is tall claim and against the waves but it is the reality! 🙂

प्राणमय कोष is our immunity. It is our cellular intelligence. It is the one that manifests and govern cell-formation. It is the one that manifests cells with individual identity i.e. microbes rather than collective identity i.e. Body organ cell like skill cell, liver cell or neuron.

It is not just me! I know couple of researchers confirming the same fact and working hard to find more and more epigenetic links!

Our food, our thoughts – all influence our well-being. Genes are actually controlled by them!!

It is not genes, it is epigenetic!

In our guts, and in the guts of all animals, resides a robust ecosystem of microbes known as the microbiome. Consisting of trillions of organisms — bacteria, fungi and viruses — the microbiome is essential for host health, providing important services ranging from nutrient processing to immune system development and maintenance.

Now, in a study comparing mice raised in a “germ free” environment and mice raised under more typical lab conditions, scientists have identified yet another key role of the microbes that live within us: mediator of host gene expression through the epigenome, the chemical information that regulates which genes in cells are active.

Writing online Nov. 23 in the journal Molecular Cell, a team of researchers from the University of Wisconsin–Madison describes new research helping tease out the mechanics of how the gut microbiome communicates with the cells of its host to switch genes on and off. The upshot of the study, another indictment of the so-called Western diet (high in saturated fats, sugar and red meat), reveals how the metabolites produced by the bacteria in the stomach chemically communicate with cells, including cells far beyond the colon, to dictate gene expression and health in its host.


Research


Diet-Microbiota Interactions Mediate Global Epigenetic Programming in Multiple Host Tissues

http://dx.doi.org/10.1016/j.molcel.2016.10.025

Highlights

  • Gut microbiota alter host histone acetylation and methylation in multiple tissues
  • Western diet suppresses microbiota-driven SCFA production and chromatin effects
  • SCFAs recapitulate microbiota-driven chromatin and transcriptional effects

Summary

Histone-modifying enzymes regulate transcription and are sensitive to availability of endogenous small-molecule metabolites, allowing chromatin to respond to changes in environment. The gut microbiota produces a myriad of metabolites that affect host physiology and susceptibility to disease; however, the underlying molecular events remain largely unknown. Here we demonstrate that microbial colonization regulates global histone acetylation and methylation in multiple host tissues in a diet-dependent manner: consumption of a “Western-type” diet prevents many of the microbiota-dependent chromatin changes that occur in a polysaccharide-rich diet. Finally, we demonstrate that supplementation of germ-free mice with short-chain fatty acids, major products of gut bacterial fermentation, is sufficient to recapitulate chromatin modification states and transcriptional responses associated with colonization. These findings have profound implications for understanding the complex functional interactions between diet, gut microbiota, and host health.

Your Toxic Home Dust is Making you Fat

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Dust
Dust

I have discussed ample times that toxic chemicals and toxic oil painted walls are invisible demons at home. We are very unique humans who pay for the demons and invite them at home!!

We ignore Go dhuli (गोरज & गोधूलि बेला : Medicinal Dust) and raise questions on it! Can dust have impact on health? With this ignorance, we also invite toxic volatile chemicals at home and consume toxic dust!! 🙁

http://pubs.acs.org/doi/abs/10.1021/acs.est.7b01788
http://pubs.acs.org/doi/abs/10.1021/acs.est.7b01788

In this post, we discussed how Volatile organic compounds play against the health and how annual cleaning ritual during Diwali helps to reduce the impact (Deepawali Churning : Home Cleaning, lime wash and Immunity)

We also discussed how Vastu plays important role in maintaining healthy environment. (Microbes, Vastu and Pro-कल्याण Architecture)

Sun Rays in home is very critical to sanitize dust particles.

All Dharma Sastra(s) prescribes cleanliness of home as mandatory ritual.


Research


Characterization of Adipogenic Activity of House Dust Extracts and Semi-Volatile Indoor Contaminants in 3T3-L1 Cells

House dust spurs growth of fat cells in lab tests

Obesity and metabolic disorders are of great societal concern and generate significant human health care costs. Recently, attention has focused on the potential for environmental contaminants to act as metabolic disruptors. This study sought to evaluate the adipogenic activity of indoor house dust extracts and a suite of semivolatile organic chemicals (SVOCs) that are often ubiquitously detected in indoor environments. 3T3-L1 cells were exposed to extracts of indoor dust or individual SVOCs and assessed for triglyceride accumulation and preadipocyte proliferation. Ten of 11 house dust extracts exhibited significant triglyceride accumulation and/or proliferation at environmentally relevant levels (<20 μg of dust/well), and significant adipogenic activity was also exhibited by 28 of the SVOCs. Notably, pyraclostrobin, dibutyl phthalate, tert-butyl-phenyl diphenyl phosphate, and the isopropylated triaryl phosphates (ITPs) exhibited near maximal or supra-maximal triglyceride accumulation relative to the rosiglitazone-induced maximum. The adipogenic activity in house dust occurred at concentrations below EPA estimated child exposure levels, and raises concerns for human health impacts, particularly in children. Our results delineate a novel potential health threat and identify putative causative SVOCs that are likely contributing to this activity.

http://pubs.acs.org/doi/abs/10.1021/acs.est.7b01788

Epigenetics Explains How We Inherit Fear From Our Ancestors

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Pitru

Memories can be passed down to later generations through genetic switches that allow offspring to inherit the experience of their ancestors, year old research says.

We do pitru tarpan. Satisfying subtle presence of all ancestors. Through them, do we get messages to activate or deactivate specific genes so that we can leverage benefit of their experiences in same situations? I strongly believe in it.

Well, let the fools of modern science prove it. But we should not wait for them.


😀

Prepare yourself for Shradha paksha of 2014. Learn your roots and try to follow them. Forget about material benefits. Help your ancestors in their spiritual journey. Help yourself in your spiritual journey.


Research


Parental olfactory experience influences behavior and neural structure in subsequent generations

https://www.nature.com/neuro/journal/v17/n1/full/nn.3594.html

Using olfactory molecular specificity, we examined the inheritance of parental traumatic exposure, a phenomenon that has been frequently observed, but not understood. We subjected F0 mice to odor fear conditioning before conception and found that subsequently conceived F1 and F2 generations had an increased behavioral sensitivity to the F0-conditioned odor, but not to other odors. When an odor (acetophenone) that activates a known odorant receptor (Olfr151) was used to condition F0 mice, the behavioral sensitivity of the F1 and F2 generations to acetophenone was complemented by an enhanced neuroanatomical representation of the Olfr151 pathway. Bisulfite sequencing of sperm DNA from conditioned F0 males and F1 naive offspring revealed CpG hypomethylation in the Olfr151 gene. In addition, in vitro fertilization, F2 inheritance and cross-fostering revealed that these transgenerational effects are inherited via parental gametes. Our findings provide a framework for addressing how environmental information may be inherited transgenerationally at behavioral, neuroanatomical and epigenetic levels.

The Family Tree Of Phobia: Epigenetics Explains How We Inherit Fear From Our Ancestors

http://www.medicaldaily.com/family-tree-phobia-epigenetics-explains-how-we-inherit-fear-our-ancestors-264134

Fear can modify our DNA and influence the behavior of future generations, researchers find. According to a Nature Neuroscience study, when an ancestor endured a terrifying experience – and managed to survive – their genetic machinery kept note of it, manifesting as a phobia that gets passed down to subsequent kin.

To show this, researchers exposed mice to the smell of cherry-blossom in a panic-inducing situation. The so-called odor fear conditioning makes the mouse associate the cherry-blossom smell with a traumatic event to the point that a fear response occurs when they encounter the odor on its own. When female mice acquired this form of PTSD (post-traumatic stress disorder) before conception, the next two generations of mice still exhibited “behavioral sensitivity” towards the cherry-blossom odor despite never being exposed to it before, the authors report.

The study verified that this automatic fearful behavior was inherited rather than taught by finding that mice that were conceived via artificial insemination and fostered by mice they weren’t related to still exhibited this sensitivity to the cherry-blossom odor.

“The fact that these changes persisted after IVF, cross-fostering and across two generations is indicative of biological inheritance,” Brian Dias and Dr Kerry Ressler, researchers at Emory University, Atlanta, conclude in their study. “The second generation mice that we tested are a full and complete generation removed from the environmental perturbation of their parent; as such, our observations suggest a transgenerational phenomenon.”

Why GMO is inefficient and half-baked technology?

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GMO

For fetus growing in the womb, there are several influences that shape its future prakriti.

First, it is impact of शुक्र & शोणित (रज,वीर्य). Parental legacy. 7 layered generational legacy. शुक्रशोणित प्रकृति.

Second, it is timing of the conception and health of the womb that has impact on the growing fetus. For example, वर्षाषु मारुतो दुष्ट: | Conception during monsoon means influence of Vayu. कालगर्भाशय प्रकृति|

Third, it is mother’s food intake. Mental state. Activities.

Fourth, it is महाभूतविकार प्रकृति | Past life karma and past life interaction with panch-mahabhut.

Now, all these forces together shape you and me in womb.

Here, genes play mere 25% (or less) role ( शुक्रशोणित प्रकृति.)

This is the reason why GMO is highly inefficient and random technology. 75% control is with mother nature.(In fact, it is 100% mother’s rule 🙂 ) Sometimes favorable results, sometimes not. IR8 rice (Famous green revolution hero, is now zero) to btCotton : shining examples of failures.

Pitru Impact: Mice can ‘warn’ sons, grandsons of dangers via sperm

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pitru

Much of our life’s programming is done by father (and mother too) and his responses to environment.

Imperfection is human trait. So all parents are imperfect. With their own limitations, bad habits, their time’s compulsions and विकृति(s). This is the reason I keep insisting value of पितृ तर्पण. This is to get rid of parental imperfection. Both mental (mind/habits) and physical (genes). And get blessed by ancestors.


Research


Mice can ‘warn’ sons, grandsons of dangers via sperm

http://www.abc.net.au/science/articles/2013/12/02/3902972.htm

Lab mice trained to fear a particular smell can transfer the impulse to their unborn sons and grandsons through a mechanism in their sperm, a study reveals.

The research claims to provide evidence for the concept of animals “inheriting” a memory of their ancestors’ traumas, and responding as if they had lived the events themselves.

It is the latest find in the study of epigenetics, in which environmental factors are said to cause genes to start behaving differently without any change to their underlying DNA encoding.

“Knowing how ancestral experiences influence descendant generations will allow us to understand more about the development of neuropsychiatric disorders that have a transgenerational basis,” says study co-author Brian Dias of the Emory University School of Medicine in Atlanta, Georgia.

And it may one day lead to therapies that can soften the memory “inheritance”.

For the study, Dias and co-author Kerry Ressler trained mice, using foot shocks, to fear an odour that resembles cherry blossoms.

Later, they tested the extent to which the animals’ offspring startled when exposed to the same smell. The younger generation had not even been conceived when their fathers underwent the training, and had never smelt the odour before the experiment.

The offspring of trained mice were “able to detect and respond to far less amounts of odour… suggesting they are more sensitive” to it, says Ressler co-author of the study published in the journal Nature Neuroscience.

They did not react the same way to other odours, and compared to the offspring of non-trained mice, their reaction to the cherry blossom whiff was about 200 percent stronger, he says.

The scientists then looked at a gene (M71) that governs the functioning of an odour receptor in the nose that responds specifically to the cherry blossom smell.

Poisoned Inheritance

Everyone talks about pregnant mother’s diet but no one really twist ear of would-be father and demand right diet from him.

smoking-sperm-759

No married couple is sincere enough for it.

Result? Poisoned inheritance. Not mere genes! But the environmental ghost (epigenetical factors).

A lack of folate in the diet of male mice reprograms their sperm in ways that damage their offspring. Could the same be true in men?

While female is deemed pregnant after conception, male partner remains pregnant for 9 months before conception (sperms become mature in 72 days. Before it, some period is needed for cleansing of the would-be father’s body).

So if you are planning for child, start focusing on right diet for at least 9 months before you meet your partner for child.

Pregnancy begins from the thought of having child to delivery of the child. At least 18 months. 9 months for male partner’s preparation and 9 months of mother’s nurturing.

Take care!


Research


http://www.economist.com/news/science-and-technology/21591547-lack-folate-diet-male-mice-reprograms-their-sperm-ways?fsrc=nlw%7Chig%7C12-12-2013%7C7186683%7C35321167%7CAP

A lack of folate in the diet of male mice reprograms their sperm in ways that damage their offspring. Could the same be true in men?

DOCTORS recommend that women who are pregnant, or plan to be, eat plenty of green, leafy vegetables. These are a source of vitamin B9—or folate, as it is also known—a substance that helps embryos develop by encouraging the formation of the neural tube, the precursor to the brain and the spinal cord. Folate has never been recommend to putative fathers, though, for the obvious reason that, sperm apart, a father contributes nothing to the physical substance of an embryo.

If the results of research on mice, by Sarah Kimmins and her colleagues at McGill University, in Montreal, are found to apply to people too, that may need to change. For, as they report in Nature Communications, they have discovered something odd: that folate deficiency in fathers can, in rodents at least, be as debilitating for embryos as deficiency in mothers. An absence of folate when sperm are forming causes alterations in them that affect the mice which grow from the eggs those sperm fertilise. In particular, Dr Kimmins saw serious deformities of head, spine and limbs.

This damage seems to be caused by epigenetic modification, the subject of a rapidly emerging field of research. Such modification involves a process called methylation, which alters the behaviour of genes in a way that can be passed from one generation to another. Folate’s job is to regulate methylation. How epigenetic modification might cause the defects Dr Kimmins saw, she does not yet know. But when she and her team looked at changes in the methylation patterns of genes in the sperm of folate-deficient rats they found things there that might cause other phenomena, which are not apparent at birth. These include cancer, diabetes, and even autism and schizophrenia.

Relational Biology : Membranes Not Genes

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Biologists know this very well: “Embryo development (ontogeny) depends on developmental gene regulatory networks (dGRNs), but dGRNs depend on preexisting spatial anisotropies that are defined by early embryonic axes, and those axes are established long before the embryo’s dGRNs are put in place. DNA sequences do not specify the final functional forms of most membrane components. Still less does DNA specify the spatial arrangements of those components. Yet their spatial arrangements carry essential ontogenetic information. The fact that membrane patterns carry ontogenetic information that is not specified by DNA poses a problem for any theory of evolution (such as Neo-Darwinism) that attributes the origin of evolutionary novelties to changes in a genetic program—whether at the level of DNA sequences or dGRNs.”[1]

In short, development of new cells depends more on Membrane Patterns than genes at the center. Membrane Patterns are formed based on epigenetic factors and cell-cell communication.

10427354_364297697028478_1006852561013357951_n

Every one of us completely regenerates our own skin every 7 days. A cut heals itself and disappears in a week or two. Every single cell in our skeleton is replaced every 7 years. And there are many such regeneration cycles going on right now at different layers of our physical body.

At each such new beginning constantly pushed in to our microcosm Universe, there is an opportunity to repair the damaged life codes (DNA in modern science language) and streamline irregular processes.

Sheer abundances of chances presented by mother nature to regain healthy state back.

Unfortunately, we are taught in Biology that genes control us ever since Watson and Crick proposed Genes dogma. So there is apathy and doubt related to epigenetic factors (Non-genetic factors controlling and shaping our lives) not limited to stress-free mind, stress-free social environment, natural habitat, pets, animals, plants – they all play a role in helping us regaining healthy state back.

Think about it. Take care.

[1] Membrane Patterns Carry Ontogenetic Information That Is Specified Independently of DNA

http://bio-complexity.org/ojs/index.php/main/article/viewArticle/BIO-C.2014.2

 

Epigenetics: You are what your Father did before birth

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Mindless Youth - Future parent?
Mindless Youth – Future parent?

But fathers believe in fun! Parties!

What is present married couple’s attitude before progeny planning?

हेल्लो मिस्टर . डी जे, मेरे गाने पिल्ज़ प्ले
आज नो वाइन, आज नो लागा, आज पियेंगे चाम्पपियेंन
बम बम बम बूजिंग डांसिंग एंड वी क्रुज़िंग
बौंसर पंगा लेता है तोह, गोटा कीप ईट मूविंग
चार बज गए लेकिन पार्टी अभी बाकी है
ब ब ब .., विथ द बजे ऐ

“I damn don’t care! Its a Friday night!”

=================

Damn the self-centered ignorance! You spoil not only your future but also future of the Earth by passing on weak and faulty legacy genes.

A father’s lifetime experiences can be transmitted to his offspring to affect health and development. The mechanisms underlying paternal epigenetic transmission are unclear. Unlike somatic cells, there are few nucleosomes in sperm and their function in epigenetic inheritance is unknown.

No matter who you are, human, horse, dog or donkey, the biological legacy will haunt you for entire life! Fated! Destined! You must live with them and find you way of living by adjustments.

So your life under the influence of addictions, rampant life style – you gift this to your kids.

Why so much ignorance? Do you really want to gift chaos to kids?

==================

My tips for newly married:

Unlike female egg that is renewed with every mensuration cycle, male sperm takes 72 days from inception to maturation. During this 72 days, whatever you eat, drink, how you act, what you think – they all play role in shaping biological and mental destiny of your child! Not only your child, 7 generations to come!

So before you plan for a child:

1) Regularize your routine. Same time to sleep, same time wake up. Right food always!
2) No mental stress of whatsoever!
3) No addictions
4) No junk food
5) Pious simple life

All (Sacrifices?) in the service of future generation!

Think about it! Take care!

(those of us like me, who are already parents, better late than never. Take care as a grand-father!)


Research


What your father did before you were born could influence your future health

http://www.sciencealert.com/what-your-father-did-before-you-were-born-could-influence-your-future

It might not just be expectant mothers who have to pay attention to their lifestyle. Now a new study published in Science could be relevant to a growing body of research looking at ways in which the lifestyle and environment of men before they become fathers could influence the lives of their children and grandchildren.

We know that many human traits, such as weight, height, susceptibility to disease, longevity or intelligence, can be partly inherited, but researchers have so far struggled to identify the precise genetic basis for this. This may partly be due to limitations in our understanding of how genetics works, but now there is growing interest in the potential for something called epigenetics to explain this heritability.

Epigenetics refers to the information in the genome over and above that contained in the DNA sequence. This information takes a number of forms, but the most popular ones scientists have studied relate to the chemical modification (known as methylation and acetylation) of DNA and the proteins called histones that together make up the human genome.

This epigenetic information – which influences which copies of the genes in our DNA are ‘expressed’, or used – may be passed from one generation to another during reproduction. It can even persist within a lifetime in a person’s tissues and organs, even as their cells are replenished.

Disruption of histone methylation in developing sperm impairs offspring health transgenerationally

http://science.sciencemag.org/content/early/2015/10/07/science.aab2006

A father’s lifetime experiences can be transmitted to his offspring to affect health and development. The mechanisms underlying paternal epigenetic transmission are unclear. Unlike somatic cells, there are few nucleosomes in sperm and their function in epigenetic inheritance is unknown. We generated transgenic mice in which overexpression of the histone H3 lysine 4 (H3K4) demethylase LSD1/KDM1A during spermatogenesis reduced H3K4 dimethylation in sperm. KDM1A overexpression in one generation severely impaired development and survivability of offspring. These defects persisted transgenerationally in the absence of KDM1A germ line expression and were associated with altered RNA profiles in sperm and offspring. We show that epigenetic inheritance of aberrant development can be initiated by histone demethylase activity in developing sperm, without changes to DNA methylation at CpG-rich regions.

 

Fetus, Genes, Epigenetics and GM Crops

Do you use molecular structure report of your kid’s DNA to decide his/her upbringing?
Do you use his/her blood report to identify innate nature and then use it for personality development?
Do you hire geneticist to modify his/her DNA to get desired results?

No. And yet, efforts put in parenting do pay the yield, positively or negatively.

Then why do you need to change genes of the seeds? 🙂

Prakriti
Prakriti

For fetus growing in the womb, there are several influences that shape its future prakriti.

First, it is impact of शुक्र & शोणित (रज,वीर्य). Parental legacy. 7 layers of generational legacy. It is called शुक्रशोणित प्रकृति.

Second, it is timing of the conception and health of the womb that has impact on the growing fetus. For example, वर्षाषु मारुतो दुष्ट: | Conception during monsoon means influence of Vayu. It is called कालगर्भाशय प्रकृति|

Third, it is mother’s food intake. Mental state. Activities. क्षेत्र प्रकृति |

Fourth, it is महाभूतविकार प्रकृति | Past life karma and past life interaction with panch-mahabhut.

Now, all these forces together shaped you and me in womb. And they will go it forever. They will do it forever this way only! Sanatana! Nitya! Always this way!

Please note: Genes play mere 25% (or less) role ( शुक्रशोणित प्रकृति.). 75% is about environment.

It is epigenetics! Be the custodian of master senses and learn to decipher mother nature’s hints! She always wants you to receive in abundance! Lest we realize it! Be the bridge between soil and seeds. Be the instrumental! That’s it!

This is the reason why GMO is highly inefficient and random technology. 75% control is with mother nature.(In fact, it is 100% mother’s rule 🙂 ) Sometimes favorable results, sometimes not. Read the history. IR8 rice (Famous green revolution hero, is now zero) to btCotton : shining examples of failures.


Supporting Research


Shaping Variation in the Human Immune System

http://www.cell.com/trends/immunology/abstract/S1471-4906(16)30091-6

Genetic variation accounts for 20–40% of immune variation, with enrichment of gene variants associated with autoimmunity, inflammatory disease, and susceptibility to infections among the identified genetic drivers.

Among the identified intrinsic drivers of immune variation, age is the most potent, driving a shift from a precursor-biased immune status to an inflammation-biased immune status.

A strong environmental effect on immune variation is observed, as revealed by cohabitation studies, with the strongest individual driver identified to date being chronic viral infection.

 

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